Ecotrail Putrajaya flags off from Putrajaya Botanical Gardens (Taman Botani Putrajaya). Conferred as one of Asia's Most Amazing Garden by the Royal Chelsea Foundation in 2008, the Putrajaya Botanical gardens is also home to more than 750 plant specieis from 13 collections of plant themes coming from different continents.
80KM and 50KM participants are required to attend mandatory briefing prior to flag-off. Participants are encouraged to arrive at the starting point 2 hours prior to the start time of the race. Runners must arrive at the start line before the time, according to their registered distance.